Rampant Reticulation in a Rapid Radiation of Tropical Trees—Insights from Inga (Fabaceae)

Abstract

[Abstract] Evolutionary radiations underlie much of the species diversity of life on Earth, particularly within the world’s most species-rich tree flora—that of the Amazon rainforest. Hybridization occurs in many radiations, with effects ranging from homogenization of divergent species to the generation of genetic and phenotypic novelty that fuels speciation. However, the influence of hybridization on Amazonian tree radiations has been little studied. We address this using the ubiquitous, species-rich, Neotropical tree genus Inga, which typifies rapid radiations of rainforest trees. We assess patterns of gene tree incongruence to ascertain whether hybridization was associated with rapid radiation in Inga. Given the importance of insect herbivory in structuring rainforest tree communities (and hence the potential for hybridization to promote adaptation through admixture of defense traits), we also test whether introgression of loci underlying chemical defenses against herbivory occurred during the radiation of Inga. Our phylogenomic analyses of 189/288 Inga species using >1300 target capture loci showed widespread introgression in Inga. Specifically, we found widespread phylogenetic incongruence explained by introgression, with phylogenetic networks recovering multiple introgression events across Inga and up to 20% of shared, likely introgressed, genetic variation between some species. In addition, most defense chemistry loci showed evidence of positive selection and marginally higher levels of introgression. Overall, our results suggest that introgression has occurred widely over the course of Inga’s history, possibly in a syngameon scenario, likely facilitated by extensive dispersal across Amazonia. Furthermore, in some cases, introgression of chemical defense loci may influence adaptation in Inga.