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Improvement of Endothelial Function by Gunnera Tinctoria Extract With Antioxidant Properties
dc.contributor.author | Sabando, Constanza | |
dc.contributor.author | Rodríguez-Díaz, Maité | |
dc.contributor.author | Ide, Walther | |
dc.contributor.author | Pastene, Edgar | |
dc.contributor.author | Avello, Marcia | |
dc.contributor.author | Simirgiotis, Mario | |
dc.contributor.author | Rojas, Susana | |
dc.contributor.author | Villarroel, Enrique | |
dc.contributor.author | Silva-Grecchi, Tiare | |
dc.contributor.author | Gutiérrez, Cristian | |
dc.contributor.author | Bouza, Rebeca | |
dc.contributor.author | Cicchelli, Bárbara | |
dc.contributor.author | González, Marcelo | |
dc.contributor.author | Rodríguez-Llamazares, Saddys | |
dc.date.accessioned | 2020-12-15T12:50:32Z | |
dc.date.available | 2020-12-15T12:50:32Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | Sabando, C., Rodríguez-Díaz, M., Ide, W. et al. Improvement of endothelial function by Gunnera tinctoria extract with antioxidant properties. Biol Res 53, 55 (2020). https://doi.org/10.1186/s40659-020-00322-2 | es_ES |
dc.identifier.issn | 0717-6287 | |
dc.identifier.uri | http://hdl.handle.net/2183/26944 | |
dc.description.abstract | [Abstract] Background: Gunnera tinctoria has been collected by Mapuche-Pewenche people for food and medicinal purposes. The high polyphenol content of methanolic extract from G. tinctoria leaves with chemical constituents such as ellagic acid and quercetin derivatives suggests its application to prevent endothelial dysfunction and oxidative stress. The aim of this study was to provide evidence of the protective effect of this extract on endothelial function by reducing oxidative stress induced by high D-glucose and H2O2, as well as by stimulating nitric oxide (NO) levels in human umbilical vein endothelial cells (HUVECs). Results: A methanolic extract with a high content of polyphenols (520 ± 30 mg gallic acid equivalents/g dry extract) was obtained from G. tinctoria leaves. Its main constituent was ellagic acid. The results of Ferric reducing antioxidant power and 2,2-diphenyl-1-picrylhydrazyl radical scavenging assays of the extract confirmed its antioxidant activity by inhibition pathway of radical species. The incubation of HUVECs with the extract decreased the apoptosis and reactive oxygen species (ROS) synthesis induced by high extracellular concentration of D-glucose or hydrogen peroxide. The extract increased endothelial NO levels and reduced vasoconstriction in human placental vessels. Conclusions: This study provides evidence about the antioxidant and endothelial protective properties of methanolic G. tinctoria leaf extract. The extract improves the availability of NO in HUVECs, inhibiting the production of ROS and vasoconstriction. | es_ES |
dc.description.sponsorship | Comisión Nacional de Investigación Científica y Tecnológica (Chile); 1191651 | es_ES |
dc.description.sponsorship | InnovaChile; 13IDL223120 | es_ES |
dc.description.sponsorship | Gobierno Regional del Biobío (Chile); R17A10003 | es_ES |
dc.description.sponsorship | Comisión Nacional de Investigación Científica y Tecnológica (Chile); AFB170007 | es_ES |
dc.description.sponsorship | Comisión Nacional de Investigación Científica y Tecnológica (Chile); EQM150025 | es_ES |
dc.description.sponsorship | Comisión Nacional de Investigación Científica y Tecnológica (Chile); AFB170007 | es_ES |
dc.description.sponsorship | Universidad de Concepción (Chile); VRID-Asociativo 213.A84.014-1.0 | es_ES |
dc.description.sponsorship | Universidad de Concepción (Chile); VRID-Asociativo 217.033.110-1.0 | es_ES |
dc.description.sponsorship | Universidad de Concepción (Chile); VRID-Enlace 216.033.108-1.0 | es_ES |
dc.description.sponsorship | This work has been financed by Fondecyt Regular [Grant Number 1191651], InnovaChile [Grant Number 13IDL223120], CIPA, CONICYT Regional, GORE BIO BIO [Grant Number R17A10003], CONICYT PIA/APOYO CCTE [Grant Number AFB170007], Fondequip [Grant Numbers EQM150025 and AFB170007] and Universidad de Concepción, Chile [Grant Numbers VRID-Asociativo 213.A84.014-1.0, VRID-Asociativo 217.033.110-1.0, VRID-Enlace 216.033.108-1.0] | |
dc.language.iso | eng | es_ES |
dc.publisher | Springer Nature | es_ES |
dc.relation.uri | https://doi.org/10.1186/s40659-020-00322-2 | es_ES |
dc.rights | Atribución 4.0 Internacional | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Gunnera tinctoria | es_ES |
dc.subject | Methanolic extract | es_ES |
dc.subject | Endothelial function | es_ES |
dc.subject | Antioxidant activity | es_ES |
dc.title | Improvement of Endothelial Function by Gunnera Tinctoria Extract With Antioxidant Properties | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.access | info:eu-repo/semantics/openAccess | es_ES |
UDC.journalTitle | Biological Research | es_ES |
UDC.volume | 53 | es_ES |
UDC.issue | 55 | es_ES |
UDC.startPage | 1 | es_ES |
UDC.endPage | 14 | es_ES |
dc.identifier.doi | 10.1186/s40659-020-00322-2 |