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dc.contributor.authorPereira, Renato
dc.contributor.authorPereira, David
dc.contributor.authorJiménez, Carlos
dc.contributor.authorRodríguez, Jaime
dc.contributor.authorNieto, Rosa M.
dc.contributor.authorVideira, Romeu
dc.contributor.authorSilva, Olga
dc.contributor.authorAndrade, Paula B.
dc.contributor.authorValentão, Patrícia
dc.date.accessioned2019-06-26T16:02:37Z
dc.date.available2019-06-26T16:02:37Z
dc.date.issued2019-06-03
dc.identifier.citationPereira, R.B.; Pereira, D.M.; Jiménez, C.; Rodríguez, J.; Nieto, R.M.; Videira, R.A.; Silva, O.; Andrade, P.B.; Valentão, P. Anti-inflammatory effects of 5α,8α-epidioxycholest-6-en-3β-ol, a steroidal endoperoxide isolated from "Aplysia depilans", based on bioguided fractionation and NMR analysis. Mar. Drugs 17(6), 330 (2019).es_ES
dc.identifier.issn1660-3397
dc.identifier.urihttp://hdl.handle.net/2183/23308
dc.description.abstract[Abstract:] Sea hares of "Aplysia" genus are recognized as a source of a diverse range of metabolites. 5α,8α-Endoperoxides belong to a group of oxidized sterols commonly found in marine organisms and display several bioactivities, including antimicrobial, anti-tumor, and immunomodulatory properties. Herein we report the isolation of 5α,8α-epidioxycholest-6-en-3β-ol (EnP(5,8)) from "Aplysia depilans" Gmelin, based on bioguided fractionation and nuclear magnetic resonance (NMR) analysis, as well as the first disclosure of its anti-inflammatory properties. EnP(5,8) revealed capacity to decrease cellular nitric oxide (NO) levels in RAW 264.7 macrophages treated with lipopolysaccharide (LPS) by downregulation of the Nos2 (inducible nitric oxide synthase, iNOS) gene. Moreover, EnP(5,8) also inhibited the LPS-induced expression of cyclooxygenase-2 (COX-2), interleukin 6 (IL-6), and tumor necrosis factor alpha (TNF-α) at the mRNA and protein levels. Mild selective inhibition of COX-2 enzyme activity was also evidenced. Our findings provide evidence of EnP(5,8) as a potential lead drug molecule for the development of new anti-inflammatory agents.es_ES
dc.description.sponsorshipPortugal.Fundação para a Ciência e Tecnologia; UID/QUI/50006/2019es_ES
dc.description.sponsorshipPortugal. Fundação para a Ciência e Tecnologia; PD/BD/113565/2015es_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.relation.ispartofseries330
dc.relation.urihttps://doi.org/10.3390/md17060330es_ES
dc.rightsAtribución 4.0 Españaes_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/es/*
dc.subjectAplysia depilanses_ES
dc.subject5α,8α-epidioxycholest-6-en-3β-oles_ES
dc.subjectiNOSes_ES
dc.subjectCOX-1es_ES
dc.subjectCOX-2es_ES
dc.subjectIL-6es_ES
dc.subjectTNF-αes_ES
dc.titleAnti-inflammatory effects of 5α,8α-epidioxycholest-6-en-3β-ol, a steroidal endoperoxide isolated from "Aplysia depilans", based on bioguided fractionation and NMR analysises_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessinfo:eu-repo/semantics/openAccesses_ES
UDC.journalTitleMarine Drugses_ES
UDC.volume17es_ES
UDC.issue6es_ES


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