Skip navigation
  •  Inicio
  • UDC 
    • Cómo depositar
    • Políticas do RUC
    • FAQ
    • Dereitos de Autor
    • Máis información en INFOguías UDC
  • Percorrer 
    • Comunidades
    • Buscar por:
    • Data de publicación
    • Autor
    • Título
    • Materia
  • Axuda
    • español
    • Gallegan
    • English
  • Acceder
  •  Galego 
    • Español
    • Galego
    • English
  
Ver ítem 
  •   RUC
  • 1. Investigación
  • Grupos de investigación
  • Grupo Fisiopatoloxía Endócrina, Nutricional e Médica (FENM)
  • GI-FENM - Artigos
  • Ver ítem
  •   RUC
  • 1. Investigación
  • Grupos de investigación
  • Grupo Fisiopatoloxía Endócrina, Nutricional e Médica (FENM)
  • GI-FENM - Artigos
  • Ver ítem
JavaScript is disabled for your browser. Some features of this site may not work without it.

Bioinformatics approach to mRNA markers discovery for detection of circulating tumor cells in patients with gastrointestinal cancer

Thumbnail
Ver/abrir
Vlldres_Binfrmtics.pdf (637.6Kb)
Use este enlace para citar
http://hdl.handle.net/2183/20803
Atribución-NoComercial-SinDerivadas 3.0 España
A non ser que se indique outra cousa, a licenza do ítem descríbese como Atribución-NoComercial-SinDerivadas 3.0 España
Coleccións
  • INIBIC-OCT - Artigos [21]
  • GI-FENM - Artigos [96]
Metadatos
Mostrar o rexistro completo do ítem
Título
Bioinformatics approach to mRNA markers discovery for detection of circulating tumor cells in patients with gastrointestinal cancer
Autor(es)
Valladares-Ayerbes, Manuel
Díaz-Prado, Silvia
Reboredo, Margarita
Medina Villaamil, Vanessa
Iglesias-Díaz, Pilar
Lorenzo-Patiño, María J.
García Campelo, Rosario
Haz, Mar
Santamarina, Isabel
Antón-Aparicio, Luis M.
Data
2008
Cita bibliográfica
Valladares-Ayerbes M, Díaz-Prado S, Reboredo M, Medina V, Iglesias-Díaz P, Lorenzo-Patiño MJ, et al. Bioinformatics approach to mRNA markers discovery for detection of circulating tumor cells in patients with gastrointestinal cancer. Cancer Detect Prev. 2008;32(3):236-250
Resumo
[Abstract] Background: Detection of tumor cells in the blood, or minimal deposits in distant organs as bone marrow, could be important to identify cancer patients at high risk of relapse or disease progression. Quantitative polymerase chain reaction (PCR) amplification of tissue or tumor selective mRNA is the most powerful tool for the detection of this circulating or occult metastatic cells. Our study aims to identify novel gastrointestinal cancer-specific markers for circulating tumor cell detection. Method: Phase I preclinical study was performed by means of computational tools for expression analysis. In silico data were used to identify and prioritize molecular markers highly expressed in gastrointestinal cancers but absent in hematopoietic-derived libraries. Selected genes were evaluated by means of qRT-PCR in gastrointestinal cancer and hematopoietic cell-lines, normal human bone marrows and bloods, tumor tissue, and blood from cancer patients. Results: Novel and known mRNA markers for circulating tumor cell detection in gastrointestinal cancer have been identified. Among all the genes assessed, PKP3, AGR2, S100A16, S100A6, LGALS4, and CLDN3 were selected and assays based on blood qRT-PCR were developed. Reliably qRT-PCR assays for the novel targets plakophilin 3 (PKP3) and anterior gradient-2 (AGR2) to identify blood-borne cells in cancer patients were developed. Conclusions: Novel and known gastrointestinal-specific mRNA markers for circulating tumor cells have been identified through in silico analysis and validated in clinical material. qRT-PCR assay targeted to PKP3 and AGR2 mRNAs might be helpful to detect circulating tumor cells in patients with gastrointestinal cancer.
Palabras chave
Neoplasm circulating cells
Metastasis
Biological tumor markers
Reverse transcriptase polymerase chain reaction
Gene expression pattern analysis
Databases nucleic acid
Epithelial cells
Tumor cell lines
Anterior gradient 2 homolog (Xenopus laevis) protein
Plakophilin 3
 
Versión do editor
https://doi.org/10.1016/j.cdp.2008.08.002
Dereitos
Atribución-NoComercial-SinDerivadas 3.0 España
ISSN
0361-090X
1525-1500
 

Listar

Todo RUCComunidades e colecciónsPor data de publicaciónAutoresTítulosMateriasEsta colecciónPor data de publicaciónAutoresTítulosMaterias

A miña conta

AccederRexistro

Estatísticas

Ver Estatísticas de uso
Sherpa
OpenArchives
OAIster
Scholar Google
UNIVERSIDADE DA CORUÑA. Servizo de Biblioteca.    DSpace Software Copyright © 2002-2013 Duraspace - Suxestións