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dc.contributor.authorSangiao-Alvarellos, Susana
dc.contributor.authorManfredi-Lozano, María
dc.contributor.authorRuiz-Pino, Francisco
dc.contributor.authorNavarro, V.M.
dc.contributor.authorSánchez-Garrido, M.A.
dc.contributor.authorLeón, Silvia
dc.contributor.authorDiéguez, Carlos
dc.contributor.authorCordido, Fernando
dc.contributor.authorMatagne, V.
dc.contributor.authorDissen, G.A.
dc.contributor.authorOjeda, S.R.
dc.contributor.authorPinilla, Leonor
dc.contributor.authorTena-Sempere, Manuel
dc.date.accessioned2016-11-15T11:57:16Z
dc.date.available2016-11-15T11:57:16Z
dc.date.issued2013
dc.identifier.citationSangiao-Alvarellos S, Manfredi-Lozano M, Ruiz-Pino F, et al. Changes in hypothalamic expression of the Lin28/let-7 system and related MicroRNAs during postnatal maturation and after experimental manipulations of puberty. Endocrinol. 2013;154(2):942-955es_ES
dc.identifier.issn0013-722
dc.identifier.issn1945-7170
dc.identifier.urihttp://hdl.handle.net/2183/17559
dc.description.abstract[Abstract] Lin28 and Lin28b are related RNA-binding proteins that inhibit the maturation of miRNAs of the let-7 family and participate in the control of cellular stemness and early embryonic development. Considerable interest has arisen recently concerning other physiological roles of the Lin28/let-7 axis, including its potential involvement in the control of puberty, as suggested by genome-wide association studies and functional genomics. We report herein the expression profiles of Lin28 and let-7 members in the rat hypothalamus during postnatal maturation and in selected models of altered puberty. The expression patterns of c-Myc (upstream positive regulator of Lin28), mir-145 (negative regulator of c-Myc), and mir-132 and mir-9 (putative miRNA repressors of Lin28, predicted by bioinformatic algorithms) were also explored. In male and female rats, Lin28, Lin28b, and c-Myc mRNAs displayed very high hypothalamic expression during the neonatal period, markedly decreased during the infantile-to-juvenile transition and reached minimal levels before/around puberty. A similar puberty-related decline was observed for Lin28b in monkey hypothalamus but not in the rat cortex, suggesting species conservation and tissue specificity. Conversely, let-7a, let-7b, mir-132, and mir-145, but not mir-9, showed opposite expression profiles. Perturbation of brain sex differentiation and puberty, by neonatal treatment with estrogen or androgen, altered the expression ratios of Lin28/let-7 at the time of puberty. Changes in the c-Myc/Lin28b/let-7 pathway were also detected in models of delayed puberty linked to early photoperiod manipulation and, to a lesser extent, postnatal underfeeding or chronic subnutrition. Altogether, our data are the first to document dramatic changes in the expression of the Lin28/let-7 axis in the rat hypothalamus during the postnatal maturation and after different manipulations that disturb puberty, thus suggesting the potential involvement of developmental changes in hypothalamic Lin28/let-7 expression in the mechanisms permitting/leading to puberty onset.es_ES
dc.description.sponsorshipMinisterio de Economia y Competitividad; BFU 2008-00984es_ES
dc.description.sponsorshipMinisterio de Economia y Competitividad; BFU 2011-25021es_ES
dc.description.sponsorshipJunta de Andalucía; P08-CVI-03788es_ES
dc.description.sponsorshipUnited States. National Institutes of Health HD025123-ARRAes_ES
dc.description.sponsorshipUnited States. National Science Foundation; IOS1121691es_ES
dc.description.sponsorshipInstituto de Salud Carlos III; PI10/00088es_ES
dc.description.sponsorshipXunta de Galicia; IN845B-2010/187es_ES
dc.description.sponsorshipXunta de Galicia; 10CSA916014PRes_ES
dc.language.isoenges_ES
dc.publisherEndocrine Societyes_ES
dc.relation.urihttp://dx.doi.org/10.1210/en.2012-2006es_ES
dc.titleChanges in hypothalamic expression of the Lin28/let-7 system and related MicroRNAs during postnatal maturation and after experimental manipulations of pubertyes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessinfo:eu-repo/semantics/openAccesses_ES
UDC.journalTitleEndocrinologyes_ES
UDC.volume154es_ES
UDC.issue2es_ES
UDC.startPage942es_ES
UDC.endPage955es_ES


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