Mostrar o rexistro simple do ítem
Changes in hypothalamic expression of the Lin28/let-7 system and related MicroRNAs during postnatal maturation and after experimental manipulations of puberty
dc.contributor.author | Sangiao-Alvarellos, Susana | |
dc.contributor.author | Manfredi-Lozano, María | |
dc.contributor.author | Ruiz-Pino, Francisco | |
dc.contributor.author | Navarro, V.M. | |
dc.contributor.author | Sánchez-Garrido, M.A. | |
dc.contributor.author | León, Silvia | |
dc.contributor.author | Diéguez, Carlos | |
dc.contributor.author | Cordido, Fernando | |
dc.contributor.author | Matagne, V. | |
dc.contributor.author | Dissen, G.A. | |
dc.contributor.author | Ojeda, S.R. | |
dc.contributor.author | Pinilla, Leonor | |
dc.contributor.author | Tena-Sempere, Manuel | |
dc.date.accessioned | 2016-11-15T11:57:16Z | |
dc.date.available | 2016-11-15T11:57:16Z | |
dc.date.issued | 2013 | |
dc.identifier.citation | Sangiao-Alvarellos S, Manfredi-Lozano M, Ruiz-Pino F, et al. Changes in hypothalamic expression of the Lin28/let-7 system and related MicroRNAs during postnatal maturation and after experimental manipulations of puberty. Endocrinol. 2013;154(2):942-955 | es_ES |
dc.identifier.issn | 0013-722 | |
dc.identifier.issn | 1945-7170 | |
dc.identifier.uri | http://hdl.handle.net/2183/17559 | |
dc.description.abstract | [Abstract] Lin28 and Lin28b are related RNA-binding proteins that inhibit the maturation of miRNAs of the let-7 family and participate in the control of cellular stemness and early embryonic development. Considerable interest has arisen recently concerning other physiological roles of the Lin28/let-7 axis, including its potential involvement in the control of puberty, as suggested by genome-wide association studies and functional genomics. We report herein the expression profiles of Lin28 and let-7 members in the rat hypothalamus during postnatal maturation and in selected models of altered puberty. The expression patterns of c-Myc (upstream positive regulator of Lin28), mir-145 (negative regulator of c-Myc), and mir-132 and mir-9 (putative miRNA repressors of Lin28, predicted by bioinformatic algorithms) were also explored. In male and female rats, Lin28, Lin28b, and c-Myc mRNAs displayed very high hypothalamic expression during the neonatal period, markedly decreased during the infantile-to-juvenile transition and reached minimal levels before/around puberty. A similar puberty-related decline was observed for Lin28b in monkey hypothalamus but not in the rat cortex, suggesting species conservation and tissue specificity. Conversely, let-7a, let-7b, mir-132, and mir-145, but not mir-9, showed opposite expression profiles. Perturbation of brain sex differentiation and puberty, by neonatal treatment with estrogen or androgen, altered the expression ratios of Lin28/let-7 at the time of puberty. Changes in the c-Myc/Lin28b/let-7 pathway were also detected in models of delayed puberty linked to early photoperiod manipulation and, to a lesser extent, postnatal underfeeding or chronic subnutrition. Altogether, our data are the first to document dramatic changes in the expression of the Lin28/let-7 axis in the rat hypothalamus during the postnatal maturation and after different manipulations that disturb puberty, thus suggesting the potential involvement of developmental changes in hypothalamic Lin28/let-7 expression in the mechanisms permitting/leading to puberty onset. | es_ES |
dc.description.sponsorship | Ministerio de Economia y Competitividad; BFU 2008-00984 | es_ES |
dc.description.sponsorship | Ministerio de Economia y Competitividad; BFU 2011-25021 | es_ES |
dc.description.sponsorship | Junta de Andalucía; P08-CVI-03788 | es_ES |
dc.description.sponsorship | United States. National Institutes of Health HD025123-ARRA | es_ES |
dc.description.sponsorship | United States. National Science Foundation; IOS1121691 | es_ES |
dc.description.sponsorship | Instituto de Salud Carlos III; PI10/00088 | es_ES |
dc.description.sponsorship | Xunta de Galicia; IN845B-2010/187 | es_ES |
dc.description.sponsorship | Xunta de Galicia; 10CSA916014PR | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Endocrine Society | es_ES |
dc.relation.uri | http://dx.doi.org/10.1210/en.2012-2006 | es_ES |
dc.title | Changes in hypothalamic expression of the Lin28/let-7 system and related MicroRNAs during postnatal maturation and after experimental manipulations of puberty | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.access | info:eu-repo/semantics/openAccess | es_ES |
UDC.journalTitle | Endocrinology | es_ES |
UDC.volume | 154 | es_ES |
UDC.issue | 2 | es_ES |
UDC.startPage | 942 | es_ES |
UDC.endPage | 955 | es_ES |
Ficheiros no ítem
Este ítem aparece na(s) seguinte(s) colección(s)
-
INIBIC-EENM - Artigos [51]
-
GI-FENM - Artigos [105]