Chlamydomonas Reinhardtii Cells Adjust the Metabolism to Maintain Viability in Response to Atrazine Stress
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Chlamydomonas Reinhardtii Cells Adjust the Metabolism to Maintain Viability in Response to Atrazine StressData
2015-05-19Cita bibliográfica
Esperanza, M., Seoane, M., Rioboo, C., Herrero, C., & Cid, A. (2015). Chlamydomonas reinhardtii cells adjust the metabolism to maintain viability in response to atrazine stress. Aquatic Toxicology, 165, 64-72. http://dx.doi.org/10.1016/j.aquatox.2015.05.012
Resumo
[Abstract] Chlamydomonas reinhardtii cells were exposed to a sublethal concentration of the widespread herbicide
atrazine for 3 and 24 h. Physiological parameters related to cellular energy status, such as cellular activity
and mitochondrial and cytoplasmic membrane potentials, monitored by flow cytometry, were altered
in microalgal cells exposed to 0.25 M of atrazine. Transcriptomic analyses, carried out by RNA-Seq
technique, displayed 12 differentially expressed genes between control cultures and atrazine-exposed
cultures at both tested times. Many cellular processes were affected, but the most significant changes
were observed in genes implicated in amino acid catabolism and respiratory cellular process. Obtained
results suggestthatphotosynthesis inhibitionby atrazine leads cells to get energy throughaheterotrophic
metabolism to maintain their viability.
Palabras chave
RNA-Seq
Flow cytometry
Atrazine
Microalga
Cytotoxicity
Chlamydomonas reinhardtii
Flow cytometry
Atrazine
Microalga
Cytotoxicity
Chlamydomonas reinhardtii
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ISSN
1879-1514