Browsing by Author "Bou, Germán"
Now showing items 1-20 of 76
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A D-Alanine auxotrophic live vaccine is effective against lethal infection caused by Staphylococcus aureus
Moscoso, Miriam; García, Patricia; Cabral, María P.; Rumbo, Carlos; Bou, Germán (Taylor & Francis, 2018-02-27)[Abstract] Staphylococcus aureus infections are becoming a major global health issue due to the rapid emergence of multidrug-resistant strains. Therefore, there is an urgent need to develop an effective vaccine to prevent ... -
A highly-safe live auxotrophic vaccine protecting against disease caused by non-typhoidal Salmonella Typhimurium in mice
García, Patricia; Moscoso, Miriam; Fuentes-Valverde, Victor; Rodicio, María del Rosario; Herrera-León, Silvia; Bou, Germán (Elsevier, 2022-10-18)[Abstract] Background: Salmonella enterica serovar Typhimurium (S. Typhimurium) has become an important intestinal pathogen worldwide and is responsible for lethal invasive infections in populations at risk. There is at ... -
A live auxotrophic vaccine confers mucosal immunity and protection against lethal pneumonia caused by pseudomonas aeruginosa
Cabral, María P.; Correia, Alexandra; Vilanova, Manuel; Gärtner, Fátima; Moscoso, Miriam; García, Patricia; Vallejo, Juan A.; Pérez, Astrid; Francisco-Tomé, Mónica; Fuentes-Valverde, Víctor; Bou, Germán (PLoS, 2020-02-10)[Abstract] Pseudomonas aeruginosa is one of the leading causes of nosocomial pneumonia and its associated mortality. Moreover, extensively drug-resistant high-risk clones are globally widespread, presenting a major challenge ... -
A New and Efficient Enrichment Method for Metagenomic Sequencing of Monkeypox Virus
Aja-Macaya, Pablo; Rumbo-Feal, Soraya; Poza, Margarita; Cañizares Castellanos, Angelina; Vallejo, J.A.; Bou, Germán (Springer, 2022-01-17)[Abstract] Background The methodology described in previous literature for Monkeypox virus (MPXV) sequencing shows low efficiency when using metagenomic approaches. The aim of the present study was to evaluate a new ... -
A new live auxotrophic vaccine induces cross-protection against klebsiella pneumoniae infections in mice
Moscoso, Miriam; Vallejo, Juan A.; Cabral, María P.; García, Patricia; Fuentes-Valverde, Víctor; Gato, Eva; Arca-Suárez, Jorge; Aja-Macaya, Pablo; Bou, Germán (MDPI, 2022-06-16)[Abstract] The development of a whole-cell vaccine from bacteria auxotrophic for D-amino acids present in the bacterial cell wall is considered a promising strategy for providing protection against bacterial infections. ... -
Activity and resistance mechanisms of the third generation tetracyclines tigecycline, eravacycline and omadacycline against nationwide Spanish collections of carbapenemase-producing Enterobacterales and Acinetobacter baumannii
García, Patricia; Guijarro-Sánchez, Paula; Lasarte-Monterrubio, Cristina; Muras, Andrea; Alonso-García, Isaac; Outeda-García, Michelle; Maceiras, Romina; Fernández-López, María del Carmen; Rodríguez-Coello, Arianna; García-Pose, Andrea; Blanco-Martín, Tania; González-Pinto, Lucía; Arca-Suárez, Jorge; Vázquez-Ucha, Juan Carlos; Bou, Germán; Beceiro, Alejandro (Elsevier, 2024-12-02)[Abstract] Introduction: The rise in multidrug-resistant bacteria challenges clinical microbiology. Tigecycline, eravacycline, and omadacycline show promise against carbapenem-resistant Enterobacterales and Acinetobacter ... -
Activity of cefiderocol and innovative β-lactam/β-lactamase inhibitor combinations against isogenic strains of Escherichia coli expressing single and double β-lactamases under high and low permeability conditions
Blanco-Martín, Tania; Alonso-García, Isaac; González-Pinto, Lucía; Outeda-García, Michelle; Guijarro-Sánchez, Paula; López-Hernández, Inmaculada; Pérez-Vázquez, María; Aracil, Belén; López-Cerero, Lorena; Fraile-Ribot, Pablo; Oliver, Antonio; Vázquez-Ucha, Juan Carlos; Beceiro, Alejandro; Bou, Germán; Arca-Suárez, Jorge (Elsevier, 2024-04-16)[Abstract] Objectives: To analyse the impact of the most clinically relevant β-lactamases and their interplay with low outer membrane permeability on the activity of cefiderocol, ceftazidime/avibactam, aztreonam/avibactam, ... -
Activity of imipenem, meropenem, cefepime, and sulbactam in combination with the β-lactamase inhibitor LN-1-255 against acinetobacter spp
Lasarte-Monterrubio, Cristina; Vázquez-Ucha, Juan Carlos; Maneiro, María; Arca-Suárez, Jorge; Alonso, Isaac; Guijarro-Sánchez, Paula; Buynak, John D.; Bou, Germán; González-Bello, Concepción; Beceiro, Alejandro (MDPI, 2021-02-20)[Abstract] Treatment of infections caused by Acinetobacter spp., particularly A. baumannii, is a major clinical problem due to its high rates of antibiotic resistance. New strategies must be developed; therefore, restoration ... -
Advancements in the fight against globally distributed OXA-48 carbapenemase: evaluating the new generation of carbapenemase inhibitors
Outeda-García, Michelle; Arca-Suárez, Jorge; Lence, Emilio; Rodríguez-Coello, Arianna; Maceiras, Romina; Blanco-Martín, Tania; Guijarro-Sánchez, Paula; González-Pinto, Lucía; Alonso-García, Isaac; García-Pose, Andrea; Muras, Andrea; Rodríguez-Pallares, Salud; Lasarte-Monterrubio, Cristina; González-Bello, Concepción; Vázquez-Ucha, Juan Carlos; Bou, Germán; Beceiro, Alejandro (American Society of Microbiology, 2025-01-10)[Abstract] Carbapenemase OXA-48 and its variants pose a serious threat to the development of effective treatments for bacterial infections. OXA-48-producing Enterobacterales are the most prevalent carbapenemase-producing ... -
Analysis of the degradation of broad-spectrum cephalosporins by OXA-48-producing enterobacteriaceae using MALDI-TOF MS
Oviaño, Marina; Rodicio, María del Rosario; Heinisch, Jürgen J.; Rodicio, Rosaura; Bou, Germán; Fernández, Javier (MDPI, 2019-11-26)[Abstract] The objective of the study was to evaluate the activity of OXA-48 against different broad-spectrum cephalosporins and to identify the reaction products by MALDI-TOF MS. The action of OXA-48 on cefotaxime, ... -
Anti-adhesive activity of a vaccinium corymbosum polyphenolic extract targeting intestinal colonization by klebsiella pneumoniae
Gato, Eva; Rosalowska, Alicjia; Martínez-Guitián, Marta; Lores, Marta; Bou, Germán; Pérez, Astrid (Elsevier, 2020-10-28)[Abstract] The therapeutic effect of Vaccinium polyphenols against uropathogens has been widely studied. Most attention has focused on the antimicrobial activity against P-fimbriated Escherichia coli strains. The present ... -
Anti-inflammatory and antimicrobial efficacy of coconut oil for periodontal pathogens: a triple-blind randomized clinical trial
Pardiñas-López, Simón; García-Caro, Mónica E.; Vallejo, Juan A.; Aja-Macaya, Pablo; Conde-Pérez, Kelly; Nión-Cabeza, Paula; Khouly, Ismael; Bou, Germán; Rodríguez-Cendal, Ana Isabel; Díaz-Prado, Silvia; Poza, Margarita (Springer Nature, 2025-03-14)[Abstract] Objectives: To evaluate the effect of coconut oil on the oral bacteriome and inflammatory response in patients with periodontitis by integrating next-generation sequencing analyses of pathogenic bacterial ... -
Antibiotic selection in the treatment of acute invasive infections by Pseudomonas aeruginosa: Guidelines by the Spanish Society of Chemotherapy
Mensa, José; Barberán, José; Soriano, Alex; Llinares, Pedro; Marco, Francesc; Cantón, Rafael; Bou, Germán; González del Castillo, Juan; Maseda, Emilio; Azanza, José Ramón; Pasquau, Juan; García-Vidal, Carolina; Reguera, José María; Sousa, Dolores; Gómez, Joaquín; Montejo, Miguel; Borges, Marcio; Torres, Antonio; Álvarez-Lerma, Francisco; Salavert, Miguel; Zaragora, Rafael; Oliver, Antonio (Sociedad Española de Quimioterapia, 2018-02)[Abstract] Pseudomonas aeruginosa is characterized by a notable intrinsic resistance to antibiotics, mainly mediated by the expression of inducible chromosomic β-lactamases and the production of constitutive or inducible ... -
Bioinformatics approaches to the study of antimicrobial resistance
Seoane, Alejandro; Bou, Germán (Sociedad Española de Quimioterapia, 2021-09-30)[Abstract] Detection and monitoring of antimicrobial resistance are two pillars on which clinical microbiology will be based in the coming decades. The implementation of certain technologies such as whole genome sequencing ... -
Building-Scale Wastewater-Based Epidemiology for SARS-CoV-2 Surveillance at Nursing Homes in A Coruña, Spain
Trigo-Tasende, Noelia; Vallejo, J.A.; Rumbo-Feal, Soraya; Conde-Pérez, Kelly; Nasser-Ali, Mohammed; Tarrío-Saavedra, Javier; Barbeito, Inés; Cao, Ricardo; Ladra, Susana; Bou, Germán; Poza, Margarita (MDPI, 2023-11)[Abstract] Wastewater-based epidemiology (WBE) has become an effective tool in the surveillance of infectious diseases such as COVID-19. In this work, we performed a brief study of monitoring the SARS-CoV-2 viral load ... -
CARB-ES-19 multicenter study of carbapenemase-producing klebsiella pneumonia e and escherichia coli from all spanish provinces reveals interregional spread of high-risk clones such as ST307/OXA-48 and ST512/KPC-3
Cañada, Javier E.; Moure, Zaira; Sola-Campoy, Pedro J.; Delgado-Valverde, Mercedes; Cano, María E.; Gijón, Desiree; González, Mónica; Gracia-Ahufinger, Irene; Larrosa, Nieves; Mulet, Xavier; Pitart, Cristina; Rivera, Alba; Bou, Germán; Calvo, Jorge; Cantón, Rafael; González-López, Juan José; Martínez-Martínez, Luis; Navarro, Ferrán; Oliver, Antonio; Palacios-Baena, Zaira R.; Pascual, Álvaro; Ruiz-Carrascoso, Guillermo; Vila, Jordi; Aracil, Belén; Pérez-Vázquez, María; Oteo-Iglesias, Jesús (Frontiers Media, 2022-06-30)[Abstract] Objectives: CARB-ES-19 is a comprehensive, multicenter, nationwide study integrating whole-genome sequencing (WGS) in the surveillance of carbapenemase-producing K. pneumoniae (CP-Kpn) and E. coli (CP-Eco) ... -
Carbapenem Resistance in Acinetobacter nosocomialis and Acinetobacter junii Conferred by Acquisition of blaOXA-24/40 and Genetic Characterization of the Transmission Mechanism between Acinetobacter Genomic Species
Lasarte-Monterrubio, Cristina; Guijarro, Paula; Bellés, Alba; Vázquez-Ucha, Juan Carlos; Arca-Suárez, Jorge; Fernández-Lozano, Carlos; Bou, Germán; Beceiro, Alejandro (American Society for Microbiology, 2022)[Abstract] Carbapenem resistance is increasing among Gram-negative bacteria, including the genus Acinetobacter. This study aimed to characterize, for the first time, the development of carbapenem resistance in clinical ... -
Clinical management of cUTI, cIAI, and HABP/VABP attributable to carbapenem-resistant Gram-negative infections in Spain
Ferrer, Ricard; Fariñas, María del Carmen; Maseda, Emilio; Salavert, Miguel; Bou, Germán; Díaz-Regañón, Jazmín; López, Diego; Lozano, Virginia; Gómez-Ulloa, David; Fenoll, Raquel; Lara, Nuria; McCann, Eilish (Sociedad Española de Quimioterapia, 2021-11-22)[Abstract] Objective: Carbapenem-resistant Gram-negative (CRGN) infections are a major public health problem in Spain, often implicated in complicated, healthcare-associated infections that require the use of potentially ... -
Combined use of the Ab105-2φΔCI lytic mutant phage and different antibiotics in clinical isolates of multi-resistant acinetobacter baumannii
Blasco, Lucía; Ambroa, Antonio; López, María; Fernández-García, Laura; Bleriot, Inés; Trastoy, Rocío; Ramos-Vivas, José; Coenye, Tom; Fernández-Cuenca, Felipe; Vila, Jordi; Martínez-Martínez, Luis; Rodríguez-Baño, Jesús; Pascual, Álvaro; Cisneros, José MIguel; Pachón, Gerónimo; Bou, Germán; Tomás, María (MDPI, 2019-11-12)[Abstract] Phage therapy is an abandoned antimicrobial therapy that has been resumed in recent years. In this study, we mutated a lysogenic phage from Acinetobacter baumannii into a lytic phage (Ab105-2phiΔCI) that displayed ... -
CRISPR-Cas, a Revolution in the Treatment and Study of ESKAPE Infections: Pre-Clinical Studies
González de Aledo, Manuel; González-Bardanca, Mónica; Blasco, Lucía; Pacios Santamaría, Olga; Bleriot Rial, Ines; Fernández-García, Laura; Fernández-Quejo, Melisa; López, María; Bou, Germán; Tomás, María (MDPI, 2021-06-22)[Abstract] One of the biggest threats we face globally is the emergence of antimicrobial-resistant (AMR) bacteria, which runs in parallel with the lack in the development of new antimicrobials. Among these AMR bacteria ...
