Probing Tyrosine Nitration with a Small TbIII-Metallopeptide
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Probing Tyrosine Nitration with a Small TbIII-MetallopeptideData
2023-06-06Cita bibliográfica
Sánchez-Fernández, R., Sánchez-Temprano, A., Esteban-Gómez, D., Pazos, E. (2023). Probing Tyrosine Nitration with a Small TbIII-Metallopeptide. ChemBioChem, 24, e202300072. https://doi.org/10.1002/cbic.202300072
Resumo
[Abstract] Tyrosine nitration, a post-translational modification (PTM) that takes place under nitrosative stress conditions, occurs through a non-enzymatic peroxynitrite-mediated reaction. Although protein nitration has long been considered an irreversible PTM involved in nitrosative stress-associated diseases, it has also been suggested to be a regulatory mechanism of signal transduction. Therefore, the development of tools that help to understand this protein modification is of great interest. Herein, we explore a TbIII-chelating metallopeptide to monitor tyrosine nitration. The luminescence of this probe decreases significantly between its non-nitrated and nitrated states, and this reduction in the luminescence intensity is directly related to the degree of tyrosine nitration after treatment with peroxynitrite. Remarkably, the luminescence intensity changes after nitration are not affected in the presence of complex biological media, which makes it a promising tool for understanding this protein modification.
Palabras chave
Lanthanides
Luminescent probes
Metallopeptides
Nitrosative stress
Protein tyrosine nitration
Luminescent probes
Metallopeptides
Nitrosative stress
Protein tyrosine nitration
Descrición
Financiado para publicación en acceso aberto: Universidade da Coruña/CISUG
Versión do editor
Dereitos
Atribución 4.0 Internacional
ISSN
1439-7633