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Study of hydrogen sulfide biosynthesis in synovial tissue from diabetes-associated osteoarthritis and its influence on macrophage phenotype and abundance
dc.contributor.author | Lendoiro-Cino, Natalia | |
dc.contributor.author | Rodríguez-Coello, Arianna | |
dc.contributor.author | Saborido, Anna | |
dc.contributor.author | Burguera, Elena F. | |
dc.contributor.author | Fernández-Rodríguez, Jennifer A. | |
dc.contributor.author | Meijide-Faílde, Rosa | |
dc.contributor.author | Blanco García, Francisco J | |
dc.contributor.author | Vaamonde-García, Carlos | |
dc.date.accessioned | 2023-06-30T10:55:10Z | |
dc.date.available | 2023-06-30T10:55:10Z | |
dc.date.issued | 2023-06-19 | |
dc.identifier.citation | Lendoiro-Cino N, Rodríguez-Coello A, Saborido A, F-Burguera E, Fernández-Rodríguez JA, Meijide-Faílde R, Blanco FJ, Vaamonde-García C. Study of hydrogen sulfide biosynthesis in synovial tissue from diabetes-associated osteoarthritis and its influence on macrophage phenotype and abundance. J Physiol Biochem. 2023 Aug;79(3):653-667. | es_ES |
dc.identifier.issn | 1138-7548 | |
dc.identifier.uri | http://hdl.handle.net/2183/33264 | |
dc.description.abstract | [Abstract] Type 2 diabetes (DB) is an independent risk factor for osteoarthritis (OA). However, the mechanisms underlying the connection between both diseases remain unclear. Synovial macrophages from OA patients with DB present a marked pro-inflammatory phenotype. Since hydrogen sulphide (H2S) has been previously described to be involved in macrophage polarization, in this study we examined H2S biosynthesis in synovial tissue from OA patients with DB, observing a reduction of H2S-synthetizing enzymes in this subset of individuals. To elucidate these findings, we detected that differentiated TPH-1 cells to macrophages exposed to high levels of glucose presented a lower expression of H2S-synthetizing enzymes and an increased inflammatory response to LPS, showing upregulated expression of markers associated with M1 phenotype (i.e., CD11c, CD86, iNOS, and IL-6) and reduced levels of those related to M2 fate (CD206 and CD163). The co-treatment of the cells with a slow-releasing H2S donor, GYY-4137, attenuated the expression of M1 markers, but failed to modulate the levels of M2 indicators. GYY-4137 also reduced HIF-1α expression and upregulated the protein levels of HO-1, suggesting their involvement in the anti-inflammatory effects of H2S induction. In addition, we observed that intraarticular administration of H2S donor attenuated synovial abundance of CD68+ cells, mainly macrophages, in an in vivo model of OA. Taken together, the findings of this study seem to reinforce the key role of H2S in the M1-like polarization of synovial macrophages associated to OA and specifically its metabolic phenotype, opening new therapeutic perspectives in the management of this pathology. | es_ES |
dc.description.sponsorship | This research has been funded by Instituto de Salud Carlos III through the projects PI19/01206, RETIC-RIER RD16/0012/0002, and RICORS-REI RD21/0002/0009 (co-funded by the European Regional Development Fund/European Social Fund; A way to make Europe/Investing in your future), and also by grants ED431B 2020/55 (Grupos con Potencial de Crecemento 2020) and IN607A 2021/7 (Grupos de Referencia Competitiva) from Xunta de Galicia. | |
dc.description.sponsorship | info:eu-repo/grantAgreement/ISCIII/Programa Estatal de Generación de Conocimiento y Fortalecimiento del Sistema Español de I+D+I/PI19%2F01206/ES/VALIDACION CLINICA DE NUEVOS BIOMARCADORES PREDICTIVOS DE DIAGNOSTICO Y PRONOSTICO EN ARTROSIS: EL PROYECTO HPP | |
dc.description.sponsorship | info:eu-repo/grantAgreement/MINECO/Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/RD16%2F0012%2F0002/ES/Red de Investigación en Inflamación y Enfermedades Reumáticas (RIER) | |
dc.description.sponsorship | Instituto de Salud Carlos III; RD21/0002/0009 | |
dc.description.sponsorship | Xunta de Galicia; ED431B2020/55 | |
dc.description.sponsorship | Xunta de Galicia; IN607A2021/7 | |
dc.language.iso | eng | es_ES |
dc.publisher | Springer | es_ES |
dc.relation.uri | https://doi.org/10.1007/s13105-023-00968-y | es_ES |
dc.rights | Creative Commons Attribution 4.0 International Licence (CC-BY 4.0) | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Glucose stress | es_ES |
dc.subject | Heme oxygenase-1 | es_ES |
dc.subject | Hydrogen sulfide | es_ES |
dc.subject | Macrophages | es_ES |
dc.subject | Osteoarthritis; Type 2 diabetes | es_ES |
dc.title | Study of hydrogen sulfide biosynthesis in synovial tissue from diabetes-associated osteoarthritis and its influence on macrophage phenotype and abundance | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.access | info:eu-repo/semantics/openAccess | es_ES |
UDC.journalTitle | Journal of Physiology and Biochemistry | es_ES |
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