Targeted Phospholipidomic Analysis of Synovial Fluid as a Tool for Osteoarthritis Deep Phenotyping
Use this link to cite
http://hdl.handle.net/2183/29848
Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 4.0 Internacional
Collections
- INIBIC- REUMA - Artigos [184]
Metadata
Show full item recordTitle
Targeted Phospholipidomic Analysis of Synovial Fluid as a Tool for Osteoarthritis Deep PhenotypingAuthor(s)
Date
2021-10-20Citation
Rocha B, Illiano A, Calamia V, Pinto G, Amoresano A, Ruiz-Romero C, et al. Targeted phospholipidomic analysis of synovial fluid as a tool for osteoarthritis deep phenotyping. Osteoarthr Cartil Open. 2021;3(4):100219. https://doi.org/10.1016/j.ocarto.2021.100219
Abstract
[Abstract] Objective. The aim of this study was to carry out a targeted phospholipidomic analysis on synovial fluid (SF) from patients with different grades of osteoarthritis (OA) and controls, in order to search for specific phospholipid profiles that may be useful for the deep phenotyping of this disease.
Design. Multiple reaction monitoring-mass spectrometry (MRM/MS) was applied to explore the potential phospholipidomic differences in the SF of knee OA patients (n = 15) (subclassified into early- and late-stage OA) and non-OA controls (n = 4). Multivariate statistical analyses conducted by partial least squares discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA) were performed to identify significantly altered phospholipids in OA, characterize phospholipidomic profiles associated with the radiographic stage of the disease and describe potential endotypes at early stages.
Results. Significant discrimination of phospholipid profiles between non-OA controls and the early- and late-stage OA groups were found by PLS-DA and HCA. Compared to SF from non-OA controls, OA patients showed higher levels of most quantified phospholipid species, including phosphatidylcholines (PC), phosphatidylserines and phosphatidylinositols. Furthermore, several PC species showed significant differences in abundance between the two OA subgroups and were negatively correlated with cartilage damage. Finally, two distinct endotypes of early-stage OA were identified based on the phospholipidomic profile of SF.
Conclusions. Our data provides a novel insight into the phospholipid profiles of OA synovial fluid, revealing specific alterations associated with the radiographic stage of the disease. This targeted phospholipidomic profiling also facilitated the characterization of two different OA endotypes at early stages of the disease.
Keywords
Osteoarthritis
Synovial fluid
Targeted lipidomic analysis
Phospholipids
Phenotyping
Synovial fluid
Targeted lipidomic analysis
Phospholipids
Phenotyping
Editor version
Rights
Atribución-NoComercial-SinDerivadas 4.0 Internacional
ISSN
2665-9131