Proteomic analysis of synovial fibroblasts and articular chondrocytes co-cultures reveals valuable VIP-modulated inflammatory and degradative proteins in osteoarthritis
Ver/ abrir
Use este enlace para citar
http://hdl.handle.net/2183/28605
A non ser que se indique outra cousa, a licenza do ítem descríbese como Creative Commons Attribution 4.0 International License (CC-BY 4.0)
Coleccións
- INIBIC- REUMA - Artigos [184]
Metadatos
Mostrar o rexistro completo do ítemTítulo
Proteomic analysis of synovial fibroblasts and articular chondrocytes co-cultures reveals valuable VIP-modulated inflammatory and degradative proteins in osteoarthritisAutor(es)
Data
2021-06-16Cita bibliográfica
Pérez-García S, Calamia V, Hermida-Gómez T, Gutiérrez-Cañas I, Carrión M, Villanueva-Romero R, Castro D, Martínez C, Juarranz Y, Blanco FJ, Gomariz RP. Proteomic analysis of synovial fibroblasts and articular chondrocytes co-cultures reveals valuable VIP-modulated inflammatory and degradative proteins in osteoarthritis. Int J Mol Sci. 2021 Jun 16;22(12):6441.
Resumo
[Abstract] Osteoarthritis (OA) is the most common musculoskeletal disorder causing a great disability and a reduction in the quality of life. In OA, articular chondrocytes (AC) and synovial fibroblasts (SF) release innate-derived immune mediators that initiate and perpetuate inflammation, inducing cartilage extracellular matrix (ECM) degradation. Given the lack of therapies for the treatment of OA, in this study, we explore biomarkers that enable the development of new therapeutical approaches. We analyze the set of secreted proteins in AC and SF co-cultures by stable isotope labeling with amino acids (SILAC). We describe, for the first time, 115 proteins detected in SF-AC co-cultures stimulated by fibronectin fragments (Fn-fs). We also study the role of the vasoactive intestinal peptide (VIP) in this secretome, providing new proteins involved in the main events of OA, confirmed by ELISA and multiplex analyses. VIP decreases proteins involved in the inflammatory process (CHI3L1, PTX3), complement activation (C1r, C3), and cartilage ECM degradation (DCN, CTSB and MMP2), key events in the initiation and progression of OA. Our results support the anti-inflammatory and anti-catabolic properties of VIP in rheumatic diseases and provide potential new targets for OA treatment.
Palabras chave
Osteoarthritis
Synovial fibroblasts
Chondrocytes
VIP
CHI3L1
PTX3
Complement system
Decorin
Cathepsin B
MMP2
Synovial fibroblasts
Chondrocytes
VIP
CHI3L1
PTX3
Complement system
Decorin
Cathepsin B
MMP2
Versión do editor
Dereitos
Creative Commons Attribution 4.0 International License (CC-BY 4.0)
ISSN
1422-0067