In Vitro and In Vivo Assessment of the Efficacy of Bromoageliferin, an Alkaloid Isolated from the Sponge Agelas dilatata, against Pseudomonas aeruginosa
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In Vitro and In Vivo Assessment of the Efficacy of Bromoageliferin, an Alkaloid Isolated from the Sponge Agelas dilatata, against Pseudomonas aeruginosaAutor(es)
Fecha
2020-06-23Cita bibliográfica
Pech-Puch, D., Pérez-Povedano, M., Martinez-Guitian, M., Lasarte-Monterrubio, C., Vázquez-Ucha, J. C., Bou, G., … Jimenez, C. (2020). In Vitro and In Vivo Assessment of the Efficacy of Bromoageliferin, an Alkaloid Isolated from the Sponge Agelas dilatata, against Pseudomonas aeruginosa. Marine Drugs, 18(6), 326. doi:10.3390/md18060326
Resumen
[Abstract] The pyrrole-imidazoles, a group of alkaloids commonly found in marine sponges belonging to the genus Agelas, display a wide range of biological activities. Herein, we report the first chemical study of the secondary metabolites of the sponge A. dilatata from the coastal area of the Yucatan Peninsula (Mexico). In this study, we isolated eight known alkaloids from an organic extract of the sponge. We used NMR and MS analysis and comparison with existing databases to characterize the alkaloids: ageliferin (1), bromoageliferin (2), dibromoageliferin (3), sceptrin (4), nakamuric acid (5), 4-bromo-1H-pyrrole-2-carboxylic acid (6), 4,5-dibromopyrrole-2-carboxylic acid (7) and 3,7-dimethylisoguanine (8). We also evaluated, for the first time, the activity of these alkaloids against the most problematic multidrug-resistant (MDR) pathogens, i.e., the Gram-negative bacteria Pseudomonas aeruginosa, Klebsiella pneumoniae and Acinetobacter baumannii. Bromoageliferin (2) displayed significant activity against P. aeruginosa. Comparison of the antibacterial activity of ageliferins 1–3 (of similar structure) against P. aeruginosa revealed some relationship between structure and activity. Furthermore, in in vitro assays, 2 inhibited growth and biofilm production in clinical strains of P. aeruginosa. Moreover, 2 increased the survival time in an in vivo Galleria mellonella model of infection. The findings confirm bromoageliferin (2) as a potential lead for designing new antibacterial drugs.
Palabras clave
Agelas dilatata
Yucatan Peninsula
Pyrrole-imidazole alkaloids
Pseudomonas aeruginosa
Structure-activity relationships
Antibacterial
Biofilm inhibition
Galleria mellonella
Yucatan Peninsula
Pyrrole-imidazole alkaloids
Pseudomonas aeruginosa
Structure-activity relationships
Antibacterial
Biofilm inhibition
Galleria mellonella
Versión del editor
Derechos
Atribución 4.0 Internacional
ISSN
1660-3397