Type 1 Diabetes Mellitus reversal via implantation of magnetically purified microencapsulated pseudoislets
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http://hdl.handle.net/2183/23316
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Type 1 Diabetes Mellitus reversal via implantation of magnetically purified microencapsulated pseudoisletsAutor(es)
Fecha
2019-02-10Cita bibliográfica
Espona-Noguera A, Etxebarría-Elezgarai J, Sáenz del Burgo L, et al. Type 1 Diabetes Mellitus reversal via implantation of magnetically purified microencapsulated pseudoislets. Int J Pharm. 2019; 560: 65-77
Resumen
[Abstract] Microencapsulation of pancreatic islets for the treatment of Type I Diabetes Mellitus (T1DM) generates a high quantity of empty microcapsules, resulting in high therapeutic graft volumes that can enhance the host’s immune response. We report a 3D printed microfluidic magnetic sorting device for microcapsules purification with the objective to reduce the number of empty microcapsules prior transplantation. In this study, INS1E pseudoislets were microencapsulated within alginate (A) and alginate-poly-L-lysine-alginate (APA) microcapsules and purified through the microfluidic device. APA microcapsules demonstrated higher mechanical integrity and stability than A microcapsules, showing better pseudoislets viability and biological function. Importantly, we obtained a reduction of the graft volume of 77.5% for A microcapsules and 78.6% for APA microcapsules. After subcutaneous implantation of induced diabetic Wistar rats with magnetically purified APA microencapsulated pseudoislets, blood glucose levels were restored into normoglycemia (<200 mg/dL) for almost 17 weeks. In conclusion, our described microfluidic magnetic sorting device represents a great alternative approach for the graft volume reduction of microencapsulated pseudoislets and its application in T1DM disease.
Palabras clave
Microcapsule sorting
Alginate
Superparamagnetic iron oxide nanoparticles
Microfluidics
Type I Diabetes Mellitus
Alginate
Superparamagnetic iron oxide nanoparticles
Microfluidics
Type I Diabetes Mellitus
Versión del editor
Derechos
Atribución-NoComercial-SinDerivadas 4.0 España
ISSN
0378-5173