Effects of amphetamine on development of oral candidiasis in rats
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Effects of amphetamine on development of oral candidiasis in ratsAutor(es)
Data
1999-07Cita bibliográfica
Freire Garabal M, Núñez MJ, Balboa J, Rodríguez-Cobo JA, López-Paz JM, Rey-Méndez M, Suárez-Quintanilla JA, Millán JC, Mayán JM. Effects of amphetamine on development of oral candidiasis in rats. Clin Diagn Lab Immunol. 1999;6(4):530-533
Resumo
[Abstract] Experiments were conducted to evaluate the effects of amphetamine (0.4 mg/kg of body weight/day) on the
development of oral candidiasis in Sprague-Dawley rats. Animals were submitted to surgical hyposalivation in
order to facilitate the establishment and persistence of Candida albicans infection. Treatment with drugs
(placebo or amphetamine) was initiated 7 days before C. albicans inoculation and lasted until the end of the
experiments, day 15 postinoculation. Establishment of C. albicans infection was evaluated by swabbing the
inoculated oral cavity with a sterile cotton applicator on days 2 and 15 after inoculation, followed by plating
on YEPD (yeast extract-peptone-dextrose) agar. Tissue injury was determined by the quantification of the
number and type (normal or abnormal) of papillae on the dorsal tongue per microscopic field. A semiquantitative
scale was devised to assess the degree of colonization of the epithelium by fungal hyphae. Our results
show that amphetamine exacerbates C. albicans infection of the tongues of rats. Significant increases in
Candida counts, the percentage of the tongue’s surface covered with clinical lesions, the percentage of abnormal
papillae, and the colonization of the epithelium by fungal hyphae were found in amphetamine-treated rats
compared to those found in the rats injected with a placebo. The last two parameters increased in rats treated
with the placebo compared to the parameters of the untreated control rats.