Variations in the biochemical profile of the marine microalga Dunaliella tertiolecta (Butcher) cultured with different nutrient concentrations and urea as a nitrogen source
Use este enlace para citar
http://hdl.handle.net/2183/13848Coleccións
Metadatos
Mostrar o rexistro completo do ítemTítulo
Variations in the biochemical profile of the marine microalga Dunaliella tertiolecta (Butcher) cultured with different nutrient concentrations and urea as a nitrogen sourceData
1994Cita bibliográfica
Herrero C., Abalde J., Cid A., Fidalgo P., Fabregas J. Variations in the biochemical profile of the marine microalga Dunaliella-Tertiolecta (Butcher) cultured with different nutrient concentrations and urea as a nitrogen-source. Cah. Biol. Mar. 1994; 35(2):213 – 223
Resumo
[Abstract] Variations in the biochemical profile of the marine microalga Dunaliella tertiolecta (Butcher) cultured
with different nutrient concentrations and urea as a nitrogen source.
Cultures of the marine microalga Dwwliella tertiolecta were grown in a urea-based medium, using four nutrient
concentrations following a geometrical progression of order 2, expressed as x I, x2, x4 and x8, in order to obtain a
maximum biomass production and to find out its biochemical variability. The nutrient concentration had little
effect on the growth rate in the logari thmic phase, with values between 0.40 and 0.44 doublings.day-I. Maximum
cellular densities of7.53x106 and 7.96x106 cells.ml-I were obtained in x2 and x4 cultures (corresponding to 2 and
4 mM of urea). Differences in biochemical composition were found in function of the nutrient concentration.
Protein content reached maximum values in the stationary phase in x4 and x8 cultures, with 29.56 and 29.28
pg.ceW I. The maximum value of carbohydrates was obtained in xl cultures, with 14.38 pg.cell- I. Maximum lipids
per cell were obtained in the stationary phase in cultures with the higher nutrient concentration asssayed (x8), with
23.12 pg.ceWI. Cultures at the lower nutrient concentration assayed (xl) showed a nitrogen deficiency, with cells
with low protein and high carbohydrate content at the stationary phase. [Resume] Variations du profil biochimique de la microalgue marine Dunaliellatertiolecta (Butcher) cultivee avec differentes concentrations de nourriture et dans un milieu d' uree.
Afin d'obtenir les plus gran des quantites de biomasse et de trouver la variabilite biochimique de la microalgue
marine D. tertiolecta, nous avons effectue des cultures dans un milieu d'uree, sous quatre concentrations differentes
de nourriture, suivant une progress ion geometrique d' ordre (xl, x2, x4, x8). La concentration en nutriment
azote n'a guere d'effet sur Ie taux de croissance dans la phase logarithmique (0,40 it 0,44 division.jour- I). Les densites
cellulaires maximales (7,53 x 106 et 7,96 x 106 ceII.ml- l) ont ete obtenues dans les cultures x2 et x4, correspondant
respectivement it 2 et 4 mM d' uree. Des differences au ni veau de la composition biochimique ont ete
observees en fonction de la concentration en nourriture. Les contenus en proteines les plus eleves ont ete observes
dans les cultures x4 et x8, soit 29,56 et 29,28 pg. ceWI. La valeur maxima en carbohydrates a ete obtenue dans les
cultures x I (14,38 pg.ceW I). Les contenus en lipides par cellule les plus eleves ont ete observes dans les cultures
ayant les plus grandes concentrations de nourriture (x8 : 23,12 pg.ceW I). Les cultures effectuees a la plus faible
concentration en nutriment ont conduit it la production de cellules aux contenus peu eleves en pfbteines mais eleves
en carbohydrates, lies au manque d'azote.
Palabras chave
Dunaliella tertiolecta
Biochemical profile
Marine microalgae
Nutrient concentrations
Urea
Nitrogen
Culture
Biochemical profile
Marine microalgae
Nutrient concentrations
Urea
Nitrogen
Culture